Fig. 5. Glucose production in proximal tubule (PT) suspensions. WT and KO mice (n = 5-6/sex/genotype/treatment) were treated under control or acid-loading for 1-week (n = 6/group) and PT suspensions prepared, aliquoted, and stimulated with glucocorticoid and cyclic AMP to produce glucose while in a glucose-free medium for 70 minutes. Glucose in medium was measured and normalized to protein; closed circles- WT male, open circles- KO males; closed triangles- WT females; open triangles- KO females. Two-way ANOVA p-values shown in the inset with significant values (< 0.05) in bold.